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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:19117*  Cite 
Description

Electron micrograph of corn stunt spiroplasma (CSS) in the wall of the filter chamber (part of the alimentary canal) and in the hemolymph (he) of a vector leafhopper D. gelbus. Note the mainly pleiomorphic forms in host cells and the filamentous/spiral forms (asterisks) in the hemolymph. CIL:12579, from the same image group, is identical to this image but contains labels. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Additional images from this contributor are available in the Library.

Technical Details

Detailed methods: Tissue was processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Additional abbreviations: bl, basal lamina, mf = muscle fiber, S = CSS.

Biological Sources
NCBI Organism Classification
Spiroplasma kunkelii
Dalbulus gelbus
Cell Type
muscle cell
prokaryotic cell
Cellular Component
basal lamina
Biological Context
Biological Process
pathogenesis
Attribution
Names
El-Desouky Ammar
Saskia A. Hogenhout
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL19117
Archival Resource Key (ARK)
ark:/b7295/w9cil19117
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
osmium tetroxide
lead salt
uranyl salt
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 1181px ——
Y 679px ——