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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:19125*  Cite 
Description

Electron micrograph of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) in sieve elements and other phloem cells of infected maize roots. CIL:12587, from the same image group, is an unlabeled version of this image. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. Related images are available in the Library form this contributor.

Technical Details

Detailed methods: Tissue was processed for TEM by fixation in glutaraldehyde and osmium tetroxide, embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM (non-digital camera). Negatives were scanned later to generate these electronic files. Abbreviations: n = nucleus, S = CSS, v = cell vacuole, w = cell wall, xy = xylem.

Biological Sources
NCBI Organism Classification
Spiroplasma kunkelii
Zea mays
Cell Type
plant cell
CL:0000399
prokaryotic cell
Cellular Component
plant-type cell wall
plant-type vacuole
Biological Context
Biological Process
pathogenesis
Attribution
Names
El-Desouky Ammar
Saskia A. Hogenhout
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL19125
Archival Resource Key (ARK)
ark:/b7295/w9cil19125
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
osmium tetroxide
lead salt
uranyl salt
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 864px ——
Y 592px ——