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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:195*  Cite 

The distribution of the mitochondrial inner membrane protein mitofilin, visualized here with antibody to mitofilin , highlights the tubular morphology of mitochondria in mouse 3T3 fibroblasts in G1. Cells were serum-starved for 48 hours, then cultured in normal serum levels for 8 hours, before fixation in methanol and immunolabeling with the polyclonal mitofilin antibody of Jiping Zha Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 100X oil/1.3 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: Ex 560/40, Em 630/60, dichroic 595

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
permanent cell line cell
Cellular Component
integral to mitochondrial inner membrane
Biological Context
Biological Process
cristae formation
Molecular Function
structural molecule activity
Parysek, Linda
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
local accumulation of fluorescent probe
Visualization Methods
Alexa Fluor 594
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Sample Preparation
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 1300px 104nm
Y 1030px 104nm