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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:20813*  Cite 

The membrane along the collecting canal of ruptured cells shows that the excess smooth spongiome membrane forms a three-dimensional array similar to etiolated chloroplasts when a piece of wood stays on a grass lawn too long preventing photosynthesis from occurring due to the lack of sunlight. Sometimes this configuration of membranes is referred to as cubic membranes (Landh, FEBS Lett. 369:15-17, 1995). TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm. Adapted with permission from the J. Cell Sci. 112:3733-3745, 1999. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13116) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuole
contractile vacuolar membrane
intrinsic to contractile vacuolar membrane
Biological Context
Biological Process
contractile vacuole organization
contractile vacuole discharge
Richard Allen (University of Hawaii)
J. Cell Sci. 112:3733-3745, 1999
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 2012px ——
Y 2024px ——