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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:22707*  Cite 
Description

TIP activity of CLIP-170(H2)–GFP (a carboxyl truncation of the plus end microtubule tracking protein CLIP-170) in the presence of EB1 protein (a plus end microtubule tracking protein). The movie shows pseudocolored overlaid images of rhodamine-labeled microtubules (red) and GFP-tagged CLIP-170(H2) protein (green). In the presence of unlabeled EB1, CLIP-170(H2)–GFP specifically localizes to growing microtubule plus-ends and is lost during microtubule shortening phases. (Scale bar, 2 micron.) Microtubule dynamics were visualized at 22 °C using a TIRF microscope fitted on an inverted microscope (Nikon TE-2000U). Images were captured with a backilluminated electron-multiplying CCD camera (Cascade-512B, Photometrics) using time-lapse capture at 2-sec intervals for 2-color microscopy. Movie corresponds to Video S3 from Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):492-7. Epub 2009 Jan 6.

Biological Sources
Cellular Component
microtubule
microtubule plus end
EB-1
CLIP-170(H2)
Biological Context
Biological Process
microtubule polymerization or depolymerization
Attribution
Names
Ram Dixit
Brian Barnet
Jacob E. Lazaru
Mariko Tokit
Yale E. Goldman
Erika L. F. Holzbaur
Published
Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):492-7. Epub 2009 Jan 6.
Pubmed
18227130
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL22707
Archival Resource Key (ARK)
ark:/b7295/w9cil22707
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Rhodamine
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
in vitro reconstitution
Relation To Intact Cell
cell free
Dimensions
Spatial Axis Image Size Pixel Size
X 365px 0.05µm
Y 417px 0.05µm
Time 2 seconds 49