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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23052*  Cite 
Description

MAP4 is not required for plectin binding to microtubules (MTs). Electron microscopy of control human 356 fibroblasts (CIL:23051) and MAP4 immunodepleted (CIL:23052, this image) cells after staining with antibody to NH 2 terminus of MAP4. Cells were microinjected with an antibody to the COOH-terminal domain of MAP4 to remove MAP4 from MTs. 10-nm gold particles decorate MTs in control cells, but are absent in injected cells. Plectin forms bridges between intermediate filaments and MTs in both cells. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 9d in J Cell Biol. 1996 Nov;135(4):991-1007.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
human 356 fibroblasts
Cellular Component
intermediate filament
microtubule
plectin
Biological Context
Biological Process
MAP4 depletion
Attribution
Names
Tatyana M. Svitkina
Alexander B. Verkhovsky,
Gary G. Borisy
Published
J Cell Biol. 1996 Nov;135(4):991-1007
Pubmed
8922382
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL23052
Archival Resource Key (ARK)
ark:/b7295/w9cil23052
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
tannic acid
critical_point dried specimen
Relation To Intact Cell
platinum replica
Dimensions
Spatial Axis Image Size Pixel Size
X 4271px ——
Y 2658px ——