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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23600*  Cite 
Description

Free microtubule sliding in cnm67delta mutant cells expressing GFP-Tubulin (GFP-TUB1; left panel, green in right panel merge) and RFP-Spc97 (a component of the gamma-tubulin complex, marks the minus end; middle panel, red in right panel merge). Movie plays at 50x real time and is movie S10 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATa SPC97-tdimer2::KanR cnm67delta::TRP1 ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) were grown to midlog phase and mounted on agarose pads for microscopy. Images were collected on an Olympus IX70 inverted fluorescence microscope with a 100 N.A. 1.35 oil objective lens and a CoolSNAP HQ camera (Roper Scientific), using QED software (Media Cybernetics). Timelapse images were captured at 5-s intervals.

Attribution
Names
Jeffrey K. Moore
David Sept
John A. Cooper
Published
Proc Natl Acad Sci. 2009. 106: 5147-5152.
Pubmed
19279216
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL23600
Archival Resource Key (ARK)
ark:/b7295/w9cil23600
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
widefield illumination
fluorescence microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
DsRed2FP
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 384px ——
Y 129px ——
Time 5 seconds 109