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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23602*  Cite 

Free microtubule sliding in nip100deltaCAP-Gly cnm67delta double mutant cells. Movie plays at 50x real time and is movie S12. Compare with movie S11 (CIL# 23601). Data analysis for movies S11 and S12 in Figure S4B of Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATalpha NIP100deltaCAP-Gly-13myc::KanMX6 cnm67delta::TRP1 ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) expressing GFP-tubulin (GFP-TUB1) were grown to midlog phase and mounted on agarose pads for microscopy. Images were captured on an Olympus Bmax-60F microscope equipped with a 1.35NA 100× UPlanApo objective, spinning disc Confocal Scanner Unit (CSU10), Picarro Cyan laser (488 nm), and a Stanford Photonics XR-Mega10 ICCD camera, by using QED software (Media Cybernetics). Image analysis was performed by using ImageJ. Timelapse images were captured at 10-s intervals for 15 min and each image in the movie represents a composite of 9 planes separated by 500 nm.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cell Line
nip100deltaCAP-Gly cnm67delta
Cellular Component
spindle pole body
cytoplasmic microtubule
outer plaque of spindle pole body
dynein complex
dynactin complex
spindle pole
Jeffrey K. Moore
David Sept
John A. Cooper
Proc Natl Acad Sci. 2009. 106: 5147-5152.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
each frame is a composite of 9 planes
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 133px ——
Y 119px ——
Time 10 seconds 59