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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23605*  Cite 

Timelapse movie of GFP-labeled microtubules in kar9delta cells (arrested in S-phase) combined with a deletion of the CAP-Gly domain of Nip100. The number of cells in which the spindle moved from the mother cell compartment through the neck was decreased in the CAP-Gly mutant. However, in CAP-Gly mutant cells where the spindle did move through the neck, the frequency of transits of the spindle, back-and-forth through the neck was similar to wild-type cells (compare with Movies S1 and S3, CIL#s 23599, 23604). Movie plays at 50x real time and is Movie S4 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATa NIP100deltaCAP-Gly DYN1-3GFP::TRP1 kar9delta::hygB ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) expressing GFP-tubulin (GFP-TUB1) and dynein heavy chain tagged with 3GFP (DYN1-3GFP) were arrested in hydroxyurea and mounted on agarose pads for microscopy. Images were captured on an Olympus Bmax-60F microscope equipped with a 1.35NA 100× UPlanApo objective, spinning disc Confocal Scanner Unit (CSU10), Picarro Cyan laser (488 nm), and a Stanford Photonics XR-Mega10 ICCD camera, by using QED software (Media Cybernetics). Image analysis was performed by using ImageJ. Timelapse images were captured at 10-s intervals for 15 min and each image in the movie represents a composite of 9 planes separated by 500 nm.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cell Line
NIP100deltaCAP-Gly, kar9delta
Cellular Component
spindle pole body
cytoplasmic microtubule
dynein complex
dynactin complex
spindle pole
cell cortex
Jeffrey K. Moore
David Sept
John A. Cooper
Proc Natl Acad Sci. 2009. 106: 5147-5152.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
each frame is a composite of 9 planes
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 320px ——
Y 250px ——
Time 10 seconds 97