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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23608*  Cite 

Nuclear envelope and spindle dynamics when the nucleus moves into the neck of kar9delta Nip100deltaCAP-Gly double mutant cells arrested in S phase. In these cells, the spindle failed to enter the bud neck, and the entire nucleus remained within the mother compartment (compare with movies S5 and S6, CIL# 23606 and 23607). Movie plays at 100x real time and is movie S7 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATa NIP100deltaCap-Gly NUP133-tdimer2::k.l.URA3 kar9delta::hygB ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) expressing GFP-tubulin (GFP-TUB1, to mark spindle and microtubules, left panel, green in right panel merge) and RFP-NUP133 (to mark nuclear envelope, middle panel, red in right panel merge) were arrested in hydroxyurea and mounted on agarose pads for microscopy. Images were captured on an Olympus Bmax-60F microscope equipped with a 1.35NA 100× UPlanApo objective, spinning disc Confocal Scanner Unit (CSU10), Picarro Cyan laser (488 nm) and Cobolt Jive laser (561 nm), and a Stanford Photonics XR-Mega10 ICCD camera, by using QED software (Media Cybernetics). Image analysis was performed by using ImageJ. Timelapse images were captured at 20-s intervals for 10 min and each image in the movie represents a composite of 9 planes separated by 500 nm.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cell Line
NIP100deltaCAP-Gly, kar9delta
Cellular Component
spindle pole body
cytoplasmic microtubule
dynactin complex
spindle pole
nuclear pore
nuclear membrane
dynein complex
Jeffrey K. Moore
David Sept
John A. Cooper
Proc Natl Acad Sci. 2009. 106: 5147-5152.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
each frame is a composite of 9 planes
Data Qualifiers
processed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 1359px ——
Y 364px ——
Time 20 seconds 58