Licensing

This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:23610^* Cite

Description

Free microtubule sliding in cnm67delta mutant cells expressing GFP-Tubulin (GFP-TUB1; left panel, green in right panel merge) and RFP-Spc97 (a component of the gamma-tubulin complex, marks the minus end; middle panel, red in right panel merge). Movie plays at 50x real time and is movie S9 in Proc Natl Acad Sci. 2009. 106: 5147-5152.

Technical Details

S. cerevisiae (MATa SPC97-tdimer2::KanR cnm67delta::TRP1 ura3-52 lys2-801 leu2-delta1::GFP-TUB1::LEU2 his3-delta200 trp1-delta63) were grown to midlog phase and mounted on agarose pads for microscopy. Images were collected on an Olympus IX70 inverted fluorescence microscope with a 100 N.A. 1.35 oil objective lens and a CoolSNAP HQ camera (Roper Scientific), using QED software (Media Cybernetics). Timelapse images were captured at 5-s intervals.

Biological Sources

NCBI Organism Classification: Saccharomyces cerevisiae S288c
Cell Line: cnm67delta
Cellular Component: spindle pole body; cytoplasmic microtubule; outer plaque of spindle pole body; gamma-tubulin small complex, spindle pole body; inner plaque of spindle pole body

Biological Context

Biological Process: microtubule-based movement; mitotic spindle organization; microtubule cytoskeleton organization; microtubule nucleation; cell division
Molecular Function: GTPase activity; structural constituent of cytoskeleton

Attribution

Names: Jeffrey K. Moore; David Sept; John A. Cooper
Published: Proc Natl Acad Sci. 2009. 106: 5147-5152.
Pubmed: 19279216

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL23610
Archival Resource Key (ARK): ark:/b7295/w9cil23610

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: widefield illumination; fluorescence microscopy; time lapse microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP; DsRed2FP
Data Qualifiers: raw, unprocessed data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	279px	——
Y	74px	——

Time	5 seconds	61