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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24780*  Cite 

This image is one from a set of 40nm thick serial sections through part of a Golgi ribbon from a normal rat kidney cell, generated using transmission electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The images in this set were used in 3-dimensional reconstructions of the Golgi apparatus. For more information see: Ladinsky et al. (2002) Structure of the Golgi and distribution of reporter molecules at 20°C reveals the complexity of the exit compartments, Mol Biol Cell 13:2810-2825. The other images from this serial set are included in this image group.

Technical Details

Cells were grown on 100-mesh gold EM grids, and maintained at 15°C for 4 hours before plunge freezing in liquid nitrogen (BalTec HPM-010), followed by freeze-substitution (1% glutaraldehyde, 0.1% tannic acid in acetone, replaced with 4% osmium tetroxide and 0.01% uranyl acetate), then embedded in Epon-Araldite and sectioned at 40nm (UltraCut-UCT, Leica). Sections were transferred to formvar-coated copper-rhodium slot grids (EMS) and stained with 2% aqueous uranyl acetate and Reynold's lead citrate. Images were acquired at a magnification of 13,000X with an FEI Tecnai TF20 transmission EM. For additional details refer to: Mol Biol Cell 13:2810-2825.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
epithelial cell
Cell Line
Cellular Component
Golgi apparatus
Golgi cisterna
Biological Context
Biological Process
Golgi organization
Golgi vesicle budding
Golgi vesicle transport
Mark S. Ladinsky
Christine C. Wu
Shane McIntosh
J. Richard McIntosh
Kathryn E. Howell
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Spatial Axis Image Size Pixel Size
X 1078px 0.0118µm
Y 1031px 0.0118µm