Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24794^* Cite

Description

Localization of Arp2/3 complex in the lamellioidia of a Xenopus keratocytes. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24795 shows an immuno EM image of Arp2/3 localization in a Xenopus keratocyte. CIL 24796 and 24797 show corresponding images in fibroblasts. Image corresponds to Figure 3a-c from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: keratocyte
Cellular Component: lamellipodium; actin cytoskeleton

Biological Context

Biological Process: cellular macromolecule localization

Attribution

Names: Tatyana M. Svitkina; Gary G. Borisy
Published: J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed: 10352018

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL24794
Archival Resource Key (ARK): ark:/b7295/w9cil24794

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Fluorescein (FITC); Tetramethyl rhodamine (TRITC); phalloidin; primary antibody plus labeled secondary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: glutaraldehyde fixed tissue; methanol fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	699px	0.126µm
Y	1044px	0.126µm