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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:248*  Cite 
Description

The distribution of mitochondria (labeled with antibody to heat shock protein 70) in relation to intermediate filaments (labeled with vimentin antibody) 16 hours after release from serum starvation in methanol-fixed 3T3 fibroblasts. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 100X oil/1.3 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20, Em 515-565 BP, dichroic 510 2. Ex 560/40, Em 630/60, dichroic 595

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cellular Component
type III intermediate filament
mitochondrion
Attribution
Names
Linda Parysek
Trudy Aebig
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL248
Archival Resource Key (ARK)
ark:/b7295/w9cil248
Imaging
Image Type
microscopy
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
localized accumulation of fluorescent probe
Visualization Methods
Alexa Fluor 594
Alexa Fluor 488
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Sample Preparation
Methods
methanol fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1300px 104nm
Y 1030px 104nm