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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24800*  Cite 
Description

Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus keratocyte after unprotected extraction without PEG and phalloidin. Actin network at lamellipodial rear disassembled in the course of unprotected extraction, whereas front zone remained as dense as in control cells (CIL 24801). Image corresponds to Figure 6b from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
keratocyte
Cellular Component
lamellipodium
actin cytoskeleton
Biological Context
Biological Process
actin filament organization
Molecular Function
structural constituent of cytoskeleton
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24800
Archival Resource Key (ARK)
ark:/b7295/w9cil24800
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
Processing History
unprocessed raw data
Sample Preparation
Methods
permeabilized tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
dispersed cell in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 4356px ——
Y 3229px ——