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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24804*  Cite 
Description

Differential response of lamellipodial actin network to latrunculin a (LA), a binder of monomeric actin. This electron micrograph of a Xenopus keratocyte)lamellipodium treated with LA (0.1 μM for 30 min) reveals actin depletion from the lamellipodial rear. A similar image of an LA treated fibroblast is available as CIL 24805. Image corresponds to Figure 7d from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
keratocyte
Cellular Component
actin cytoskeleton
lamellipodium
Biological Context
Biological Process
branching of actin filaments
latrunculin a treatment
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24804
Archival Resource Key (ARK)
ark:/b7295/w9cil24804
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
shadowing and plating
platinum replica
Processing History
unprocessed raw data
Sample Preparation
Methods
permeabilized tissue
glutaraldehyde fixed tissue
phalloidin
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 769px 0.017µm
Y 1226px 0.017µm