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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25362*  Cite 
Description

QuickTime movie of a complete optical scan through a rabbit intestinal villus tip infected with infected with 10× the amount (4 × 108 CFU/ml) of ΔinlA L. monocytogenes (green) than shown for Wt-GFP L. monocytogenes, and counterstained with phalloidin (red) to visualize the actin cytoskeleton, and with toto-3 (blue) to visualize nuclei. Paraformaldehyde fixed intact tissue blocks were processed without prior embedding and sectioning. The samples were imaged by confocal microscopy where optical sections were taken at 0.5 micron resolution through both the cell monolayers and the intestinal villi. Z-stacks were reconstructed into three dimensions using Volocity software. Corresponds to Video 4 from PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27.

Biological Sources
NCBI Organism Classification
Oryctolagus cuniculus
Listeria monocytogenes
Cell Type
barrier epithelial cell
bacterium
Cellular Component
nucleus
actin filament
Biological Context
Biological Process
bacterial invasion at epithelial juncitons
Attribution
Names
M Pentecost
G Otto
JA Theriot
MR Amieva
Published
PLoS Pathog. 2006 Jan;2(1):e3. Epub 2006 Jan 27
Pubmed
16446782
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25362
Archival Resource Key (ARK)
ark:/b7295/w9cil25362
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
computer graphic
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
phalloidin
toto-3
EGFP
Processing History
reconstructed z-stacks
Sample Preparation
Methods
formaldehyde fixed tissue
permeabilized tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 351px ——
Y 499px ——