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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25370*  Cite 
Description

Electron micrograph of Maize mosaic virus (MMV, Rhabdoviridae) in midgut epithelial cells of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. MMV buds through nuclear membranes and accumulates in perinuclear space in cells of the midgut. The accompanying image in this image group includes labels and scale bar. Methods: Dissected insect organs were processed for TEM by fixation in glutaraldehyde and osmium tetroxide and embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM. Associated references: Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85. Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

Biological Sources
NCBI Organism Classification
Maize mosaic virus
Peregrinus maidis
Cell Type
epithelial cell
Cellular Component
host cell perinuclear region of cytoplasm
Attribution
Names
El-Desouky Ammar
Saskia A. Hogenhout
L. R. Nault
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25370
Archival Resource Key (ARK)
ark:/b7295/w9cil25370
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
detection of electrons
illumination by electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 1333px 0.0035µm
Y 938px 0.0035µm