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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25611*  Cite 

Blood-cell flow in ventral region of a Drosophila third-instar larval body. Live visualization of blood cell flow in a Nrg-GFP;PxnYFP larva viewed ventrally. Blood cells flow from anterior (Upper) to posterior as they course around and beneath the fat body and other organs. Tissue-bound blood cells attached to the body are sessile. Time between frames, 0.34 s. Total duration, 33 s. Fig. 1C was generated from individual frames of this movie. Movie is Movie S3 of Proc Natl Acad Sci (2008). 105: 10017-10022. Movies from this publication include CIL #s 25607, 25608, 25609, 25610, 25611, 25612, 25613, 25614, 25615, 25616, 25617.

Technical Details

Third-instar [w, Nrg-GFP;Pxn-Gal4, UAS-2xeYFP] larvae were mounted dorsal side up on a glass slide with the anterior and posterior ends of the larvae taped down to varying degrees to prevent locomotion and constrain body peristalsis. Larvae were imaged on a Leica MZ16FA fluorescent stereomicroscope using a Planapo 1.0× objective, and images were captured on a monochrome Leica DFC350FX digital camera. For real-time recording of larval circulation, frames were captured every ≈400 ms over a 4- to 40-s period. Images were obtained by using the Scope-Pro Advanced Acquisition plug-in, and image analysis was carried out with Image-Pro AMS ver. 5.1 Software (Media Cybernetics).

Biological Context
Biological Process
innate immune response
wound healing
Daniel T. Babcock
Amanda R. Brock
Greg S. Fish
Yan Wang
Laurent Perrin
Mark A. Krasnow
Michael J. Galko
Proc Natl Acad Sci (2008). 105: 10017-10022.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——
Time 0.325 seconds 98