Blood-cell flow in ventral region of a Drosophila third-instar larval body. Live visualization of blood cell flow in a Nrg-GFP;PxnYFP larva viewed ventrally. Blood cells flow from anterior (Upper) to posterior as they course around and beneath the fat body and other organs. Tissue-bound blood cells attached to the body are sessile. Time between frames, 0.34 s. Total duration, 33 s. Fig. 1C was generated from individual frames of this movie. Movie is Movie S3 of Proc Natl Acad Sci (2008). 105: 10017-10022. Movies from this publication include CIL #s 25607, 25608, 25609, 25610, 25611, 25612, 25613, 25614, 25615, 25616, 25617.
Third-instar [w, Nrg-GFP;Pxn-Gal4, UAS-2xeYFP] larvae were mounted dorsal side up on a glass slide with the anterior and posterior ends of the larvae taped down to varying degrees to prevent locomotion and constrain body peristalsis. Larvae were imaged on a Leica MZ16FA fluorescent stereomicroscope using a Planapo 1.0× objective, and images were captured on a monochrome Leica DFC350FX digital camera. For real-time recording of larval circulation, frames were captured every ≈400 ms over a 4- to 40-s period. Images were obtained by using the Scope-Pro Advanced Acquisition plug-in, and image analysis was carried out with Image-Pro AMS ver. 5.1 Software (Media Cybernetics).
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