This movie illustrates the early division up to the four-cell stage of a wild-type C. elegans embryo by DIC microscopy. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Additionally, in early C. elegans embryonic cell cycles, S and M phases rapidly alternate without apparent gap phases. The movie corresponds to experiments shown in Fig. 1B and Movie 2 of Mouysset et al. Movie is CIL 25629 and original data file is CIL 35124.
Eggs were extruded in M9 buffer from dissected adult worms and mounted on 2% agarose pads. Recordings were acquired at 4-s intervals with AxioCam HR or AxioCam MRc cameras mounted on Axioplan2 Imaging or Axiophot microscopes, respectively, equipped with differential interference contrast (DIC) optics (100×/1.3 Plan-Neofluar; Carl Zeiss).
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