Elongation of the dorsal trunk ectoderm closely matches that of the ventral body side, but shows very little convergence. This time-lapse movie shows the elongation of the dorsal region of Platynereis between 54 and 58.66 hours post fertilization at 26C. The movie covers a period of 280 min in 56 frames. This is Movie 3 in supporting information for Proc Natl Acad Sci (2007) 104: 2727-2732.
To label cellular outlines, embryos were incubated for 15 min in 5 μM BODIPY564/570 coupled to propionic acid (Invitrogen, Carlsbad, CA) in natural seawater, then rinsed twice in natural seawater and mounted in a 1:1 mixture of natural seawater and 7.5% MgCl2 to prevent muscular contractions. Time-lapse recordings were performed with a PerkinElmer (Wellesley, MA) Ultraview RS System (spinning disk confocal). Embryos were kept during recordings (12 frames per hour) at a constant temperature of 25C or 26C in natural seawater between slide and coverslip separated by two layers of adhesive tape and silicone paste. The chamber was sealed with mineral oil (Sigma, St. Louis, MO).
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