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Movie shows spindle assembly around DNA-beads in a Xenopus laevis egg extract. DNA beads are autofluorescent, and spindle is formed from exogenous X-rhodamine-labeled tubulin. By the end of the movie a well-defined spindle has formed.
Images were capured every 60 sec with a Hamamatsu Orca-ER cooled CCD camera using a Nikon TE2000-U stand and a 40x 1.3 NA objective lens in widefield mode. See Supplemental video 1 in Groen et al 2009, Mol Biol Cell 20:2766-2773.
Groen AC, Maresca TJ, Gatlin JC, Salmon ED, Mitchison TJ 2009 Mol Biol Cell 20:2766-2773
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