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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27147*  Cite 

Autofluorescence from unidentified pollen (mixed pollen grain w.m.; Carolina, commercial sample), presented as a maximum intensity projection obtained from a color-coded Z-stack with 200 image planes. The colors represent the z-position within the maximum intensity projection.

Technical Details

Pollen was imaged using an iMIC Andromeda Spinning Disk Confocal (TILL Photonics GmbH Objective: Olympus UPlanSApo 60X/NA1.35 CCD Camera: Imago QE (PCO). Excitation, 561nm Laser, with the following filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma, custom-made design); and emission filter, FF01-390/482/563/640 (Semrock). The colors represent the z-position within the maximum intensity projection. All image processing was done using FIJI. After recording the z-stack (see accompanying raw data in this image group), every focal plane was colorized using the full rainbow, resulting in an RGB z-stack. The maximum intensity projection then produces a colorized max intensity projection. To access FIJI go to

Biological Sources
NCBI Organism Classification
unidentified plant
Biological Context
Biological Process
Sebastian Rhode, Till Photonics
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Data Qualifiers
processed data
Sample Preparation
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 622px 0.1075µm
Y 455px 0.1075µm