Cell junctions formed between adjacent epidermal cells in Fundulus heteroclitus scales. Desmosomes line the membranes of two epidermal cells. A vast network of intermediate filaments and microfilaments are present throughout the cytoplasm and connect to the desmosomes plates. Microtubules are also visible in the cells.
Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.
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