Image shows a z-series of a mouse ES cell carrying a multi-copy alpha-globin BAC transgene array. Relative distribution of the BAC sequences, identified by DNA FISH (green) using the entire BAC as probe, versus the heterochromatic mark H3K9me3 (red), identified by immuno-staining. DAPI staining of DNA is blue. An internal, heterochromatic core is surrounded by a more peripheral BAC signal.
Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. Pixel dimensions are 0.067 microns, z-section interval is 0.2 microns. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.
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