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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:30551*  Cite 
Description

z-series image of mouse ES cells with a multi-copy alpha-globin BAC insertion. H3K9me3 (blue) marks the heterochromatic core which largely colocalizes with combined FISH signal from the lac operator repeats and BAC vector backbone (green). In contrast the BAC FISH signal (red) is more peripheral.

Technical Details

Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. xy pixel dimensions are 0.067 microns, z spacing is 0.2 microns and number of z-slices 14. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
embryonic stem cell
Cellular Component
heterochromatin
nuclear chromatin
transgene
Biological Context
Biological Process
nucleus organization
chromatin organization
Attribution
Names
Paul Sinclair
Qian Bian
Matt Plutz
Edith Heard
Andrew Belmont
Published
P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776
Pubmed
20819934
Link
Journal of Cell Biology
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL30551
Archival Resource Key (ARK)
ark:/b7295/w9cil30551
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
distribution of a specific nucleic acid sequence
Visualization Methods
EGFP
Processing History
constrained iterative deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 218px 0.067µm
X 230px 0.067µm
Z 14px 0.2µm