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CIL:30571*  Cite 

Phagocyte and budded yeast. Amoebae (D. discoideum) were plated with yeast (S. cerevisiae) in a glass bottom chamber and viewed by confocal microscopy. Fluorescence is superimposed on grey scale brightfield images. The amoebae were expressing LimEdelta-GFP to label actin filaments. The interaction between an amoeba and two budded yeast is shown. When a phagocytic cup reaches the constriction at the neck of the first budded yeast, actin accumulates there as the amoeba attempts to seal the cup. When this attempt fails, the amoeba releases the yeast. Another budded yeast is then encountered and similarly attacked, also unsuccessfully. Finally, the amoeba returns to the first yeast and this time succeeds in separating the yeast mother and bud, allowing one portion to be ingested. The time series was captured in a single focal plane at 3.94-second intervals using a Zeiss LSM 510 laser scanning confocal microscope equipped with a 63x 1.4 NA DIC objective lens. Pixel size 0.08 x 0.08 microns. The movie plays at 30x actual speed. Correspond to Movie 1 in BMC Biol. 2010 Dec 29;8:154.

Biological Sources
NCBI Organism Classification
Dictyostelium discoideum
Saccharomyces cerevisiae
Cell Type
amoeboid cell
budded yeast
Cellular Component
actin filament
phagocytic cup
Biological Context
Biological Process
Margaret Clarke
Ulrike Engel
Jennifer Giorgione
Annette Müller-Taubenberger
Jana Prassler
Douwe Veltman
Günther Gerisch
BMC Biol. 2010 Dec 29;8:154
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
single-spot confocal microscopy
bright-field microscopy
Parameters Imaged
fluorescence emission
absorption of illumination
Source of Contrast
distribution of a specific protein
differences in intrinsic optical density
Visualization Methods
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 396px 0.08µm
Y 268px 0.08µm
Time 3.94 seconds