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CIL:31201*  Cite 

Chicken DRG neurons were grown on coverslips coated with laminin/poly-L-ornithine for 2 d and stained with 0.1 µM MitoTracker red CMXRos. Images were acquired every 2 s. To reduce the size of the video, one frame for every 10 s is shown. In the video, both DIC and fluorescent images are shown. The growth cone is toward the right-hand side. The time elapsed is indicated in the top left hand corner. During the process of growth cone advance, docked mitochondria moved at a low velocity in the anterograde direction in a coherent manner. A kymograph of the movie is shown at the bottom of the screen. Time is on the y axis and distance is on the x axis. The moving bar on the right hand side of the kymograph marks the time position. This video illustrates how kymographs correspond to time-lapse movies of mitochondrial transport, Bar, 10 µm. Images were acquired on a confocal system (FV300 FluoView; Olympus) on an IX70 microsocpe using a 60×, 1.4 NA, PlanApo objective. Samples were illuminated with an argon/krypton Omnichrome 488/568/647 line laser set to standby mode, with the laser intensity set at 6%, the confocal aperture set at 5, and pixel dwell times of either 100 or 200 microsec; the light was further attenuated with neutral density filters (either 25 or 50%). Cells were observed in an enclosed flow chamber maintained at 37°C. This video corresponds to Figure 1 and video 1 from J Cell Biol. 2006 May 8;173(3):373-81.

Biological Sources
NCBI Organism Classification
Gallus gallus
Cell Type
Cell Line
dorsal root ganglion
Cellular Component
Biological Context
Biological Process
axonal transport
Kyle E. Miller
Michael P. Sheetz
J Cell Biol. 2006 May 8;173(3):373-81.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
differential interference contrast microscopy
single-spot confocal microscopy
Parameters Imaged
optical path length gradient
fluorescence emission
Source of Contrast
boundaries between regions with different refractive index
membrane potential dye accumulation
Visualization Methods
visualization of contiguous regions
CMX rosamine (Mitotracker Red)
Processing History
unprocessed raw data
line profile
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 675px 0.33µm
Y 450px 0.33µm
Time 10 seconds 61