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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:31939*  Cite 

Movie shows actin cytoskeleton in Nck-deficient cells rescued with Nck1 after stimulation with PDGF-B. In the presence of Nck1, PDFG-B stimulation induced, in addition to peripheral ruffles, very noticeable dorsal ruffles that coincided with areas of partial disassembly of actin bundles. Supporting Movie 3 for Rivera et al.

Technical Details

Nck-deficient cells (mouse embryonic fibroblast from double knockout Nck1/Nck2) were cotransfected with Nck1 (EBB containing human Nck1) and actin-GFP to visualize cytoskeletal dynamics, serum-starved for 24 h, and then stimulated with 30 ng/ml PDGF-B. Confocal images (five optical section per time point) were obtained every 12 seconds for 5 min beginning 5 min after PDGF-B stimulation using a Nikon TE2000 inverted microscope with a 60 × 1.4 NA oil-immersion objective. The confocal system was equipped with a PerkinElmer UltraView spinning microlens and an ORCA–ER Firewire CCD camera. The display rate of the movie is five frames per second.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cellular Component
actin cytoskeleton
G. M. Rivera
S. Antoku
S. Gelkop
N. Y. Shin
S. K. Hanks
T. Pawson
B. J. Mayer
Proc Natl Acad Sci. 2006, 103: 9536-9541.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
time lapse microscopy
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 450px ——
Y 375px ——
Time 12 seconds 25