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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34929*  Cite 
Description

Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell interior at cell edge (CIL 34926) of cytochalasin D-treated human 356 fibroblasts stained with ABP-280 primary antibody and 10-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal ABP-280 at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and ABP-280 (green) can be found at (CIL 34923). Image corresponds to Figure 5h from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
human 356 fibroblast
Cellular Component
actin cytoskeleton
ABP-280
cell interior
Biological Context
Biological Process
branching of actin filaments
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL34929
Archival Resource Key (ARK)
ark:/b7295/w9cil34929
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
primary antibody plus labeled secondary antibody
immuno-gold
Processing History
pseudocolored
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
permeabilized tissue
glutaraldehyde fixed tissue
phalloidin
methanol fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 787px 2.7nm
Y 661px 2.7nm