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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35146*  Cite 

This time-lapse series illustrates the early division up to the four-cell stage of a C. elegans embryo depleted of npl-4 through RNAi. The first C. elegans embryonic division of the P0 zygote is asymmetric and generates an anterior AB cell, and a smaller posterior P1 cell. These cells have different developmental fates and division timing, with AB dividing approximately 2 min before P1. Time-lapse differential interference contrast (DIC) microscopy identified that downregulation of cdc-48, ufd-1, and npl-4 increases the cell division delay of P1 in comparison to AB, leading to a prolonged three-cell stage. The series corresponds to experiments shown in Fig. 1B and Movie 5 of Mouysset et al. Movie is CIL 25632 and original data file is CIL 35146.

Technical Details

RNA interference was performed using the feeding method. L4 larvae were placed on IPTG-containing plates seeded with Escherichia coli [HT115(DE3)] expressing double-stranded RNA. Eggs were extruded in M9 buffer from dissected adult worms and mounted on 2% agarose pads. Recordings were acquired at 4-s intervals with AxioCam HR or AxioCam MRc cameras mounted on Axioplan2 Imaging or Axiophot microscopes, respectively, equipped with differential interference contrast (DIC) optics (100×/1.3 Plan-Neofluar; Carl Zeiss).

Biological Sources
NCBI Organism Classification
Caenorhabditis elegans
Cell Type
early embryonic cell
Cell Line
Cellular Component
Cdc48p-Npl4p-Ufd1p AAA ATPase complex
Biological Context
Biological Process
cell division
nucleus organization
Molecular Function
ATPase activity
Julien Mouysset
Alexandra Deichsel
Sandra Moser
Carsten Hoege
Anthony A. Hyman
Anton Gartner
Thorsten Hoppe
Proc Natl Acad Sci. 2008. 105: 12879-12884.
Proceedings National Academy of Sciences USA
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
differential interference contrast microscopy
time lapse microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 988px ——
Y 574px ——
Time 4 seconds 205