Dominant features of a nephron in the cortex of rat kidney showing f-actin localization in the glomerulus, a blood vessel, and proximal tubules. The glomerulus is the large ball at the left. The blood vessel that delivers blood to the glomerulus (Afferent arteriole) is the bright narrow tube shown both in elongated view and in cross section (upper right) that is lined with juxtaglomerular cells. The proximal convoluted tubules are cut to reveal the actin rich micro villi lining the interior surface. This thin optical section was acquired with a laser scanning confocal microscope and is a montage of a few adjacent fields imaged at high resolution.
Formaldehyde fixed rat kidney was cut by Vibratome and incubated with fluorescent labeled phalloidin, a toxin which binds to f-actin filaments. Imaging was performed using a BioRad MRC 600 laser scanning confocal microscope with a Kr/Ar laser. The microscope was a Nikon Diaphot with a 60X or 40X fixed tube length objective. Images of adjacent fields were manually stitched using Adobe Photoshop 2.5 on a Quadra Macintosh computer and may have manual contrast curves applied. Imaging performed at the Image Analysis Facility at the Albert Einstein College of Medicine.
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