Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35175^* Cite

Description

Overexpression of and arp3-GFP fusion protein in MTLn3 cells, a metastatic rat breast carcinoma line. This Z series sequence of cells in culture shows the protein throughout the cytoplasm and excluded from a few organelles and the nucleus. This MTLn3 (rat mammary adenocarcinoma) cell line is advantageous for cancer studies because the cells grow easily in tissue culture, invade artificial 3D matrices, and form tumors in live animal models.

Technical Details

BioRad Radiance 2000 laser scanning confocal microscope with Kr/Ar laser for excitation of GFP at 488 nm. Nikon Eclipse with planapo 60X NA 1.4 phase 3 infinity corrected optics. Imaged at room temperature at the Analytical Imaging Facility at the Albert Einstein College of Medicine.

Biological Sources

NCBI Organism Classification: Rattus rattus
Cell Type: permanent cell line cell
Cell Line: MTLn3
Cellular Component: cytoskeleton

Attribution

Names: Michael Cammer; Maryse Bailly

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35175
Archival Resource Key (ARK): ark:/b7295/w9cil35175

Imaging

Image Type: single-spot confocal microscopy
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	256px	0.184µm
Y	256px	0.184µm
Z	40px	0.3µm