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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35210*  Cite 

Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. A micropipette is positioned near the cells to deliver either a growth factor or control buffer. In this sequence a PDGF-AA is added and cells extend rapidly and turn to the pipette tip.

Technical Details

These data were collected as research which led to the published article Cell Physiol Biochem 2010;25:279-292. Microscopy was performed with a 20X planapo phase contrast objective on an Olympus IX 70 microscope with a SensicamHQ cooled CCD camera and a heated stage insert set to 37 degrees C. Cells were grown and serum starved for 48 h in a coverslip bottom MatTek dish. Small amounts of PDGF-AA were continuously ejected from the micropipette to create a gradient in the vicinity of growth-arrested wt MEFs. Cell movement was monitored with time lapse video microscopy, taking images at 30 second intervals. A Femtojet Micromanipulator 5171 (Eppendorf-Brinkman Instruments) and a pump (model Femtojet; Eppendorf) were used to control the position of the micropipette and the pressure required for the chemoattractant flow. Femptotip II micropipettes were positioned within 1 μm of the cover slip and pressure set at 30 to 45 hPa. Spatial scale is approximate. Imaging performed at the Image Analysis Facility of the Albert Einstein College of Medicine.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Cellular Component
Biological Context
Biological Process
cell motility
Molecular Function
receptor signaling protein tyrosine kinase activity
Søren T. Christensen
Linda Schneider
Michael Cammer
Cell Physiol Biochem 2010;25:279-292
Journal Article
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1280px 0.17µm
Y 1024px 0.17µm
Time 30 seconds 141