Freeze substituted Lilium pollen tube immunolabeled for filamentous actin and imaged using laser scanning confocal microscopy. Shown is a projection of z-slices revealing the dense sub-apical actin fringe. Other images in the group reveal the distributions of other cytoskeletal elements and membranous components.
Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-actin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of z-slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.
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