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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35288*  Cite 

Living Lilium pollen tube labeled with dichloro-flourescein diacetate to mark the vacuole and imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice.The growing tip is devoid of vacuole. Other images in the group reveal the distributions of additional cytoplasmic components.

Technical Details

Living lily pollen tubes were labeled and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Image shows a central x-y plane through the tube. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
plant-type vacuole lumen
Biological Context
Biological Process
pollen tube growth
Alenka Lovy-Wheeler
Luis Cardenas
Joseph Kunkel
Peter K Hepler
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
carboxy-dichloroflourescein diacetate
Processing History
central x-y slice
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 500px ——
Y 178px ——