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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35290*  Cite 

Freeze substituted Lilium pollen tube immunolabeled for microtubules (blue>magenta) and imaged using laser scanning confocal microscopy. Shown is a projection of x-y slices revealing the distribution of microtubules in the sub-apical region. This group of images also contains an x-z projection of the same specimen.

Technical Details

Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-tubulin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of x-y slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
Biological Context
Biological Process
pollen tube growth
Alenka Lovy-Wheeler
Kathleen L Wilsen
Tobias I Baskin
Peter K Hepler
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
distribution of epitope
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
projection of confocal slices
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
cryofixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 2001px ——
Y 424px ——