Living Lilium pollen tube labeled with paclitaxel (blue) to mark microtubules (blue) and cytochalasin D TMR to mark endoplasmic reticulum (green) imaged using laser scanning focal microscopy. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.
Living lily pollen tubes were labeled and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green (endoplasmic reticulum) and blue (microtubules) signals from a central plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.
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