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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35295*  Cite 

Living Lilium pollen tube labeled with carboxy-dichlorofluorofluorescein (green) to mark the vacuole and cytochalasin D TMR to mark endoplasmic reticulum (red) imaged using laser scanning focal microscopy. Shown is a central 1.0 micron thick x-y slice. The growing tip is devoid of both organelles. Other images in the group reveal the distributions of additional cytoplasmic components.

Technical Details

Living lily pollen tubes were labeled and then imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. Green and red signals from a central plane through the tube were merged to generate the image. See Lovy-Wheeler et al. 2007 Cell Motil Cytoskel 64:217-321.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
plant-type vacuole lumen
endoplasmic reticulum
Biological Context
Biological Process
pollen tube growth
Alenka Lovy-Wheeler
Luis Cardenas
Joseph Kunkel
Peter K Hepler
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
Tetramethyl rhodamine (TRITC)
Processing History
merged confocal slices
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 983px ——
Y 242px ——