Time-lapse microscopy of a b-Tubulin GFP expressing mouse oocyte injected with siCo-5h, a control siRNA. Images were taken every 20 minutes. Times after the resumption of meiotic maturation and germinal vesicle breakdown (GVBD) are indicated in the lower left corner. Video S3. Found at: doi:10.1371/journal.pone.0003338.s003
Time-lapse images were acquired using a Photometrics CCD camera (CoolSnap HQ2) mounted on a Leica DMI600B microscope using a Leica HC PL APO 20X/0.7 NA objective enclosed in a thermostatic chamber (Life Imaging Services). Immunofluorescence methods can be found in the cited paper and in Brunet S. et al. (1999) J Cell Biol 146: 1–11.
|Spatial Axis||Image Size||Pixel Size|