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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35450*  Cite 

First cleavage of a live mouse zygote. Clear circles are the nuclei which are in a sea of granule dense yolk. The band around the two cells is the zona pellucida.

Technical Details

Fertilized mouse egg was kept at 37 degrees Celsius in a MatTek coverslip bottom dish on an inverted microscope stage. Imaged using a Leica SP2 AOBS confocal with a 488 nm laser and 10X or 20X objective lens. The pinhole was set to Leica's calculated size for 1 Airy unit, each Z section was scanned in one pass, and the bit depth was 8. Additional information may be found in the Leica generated meta text file attached to the image. Microscopy was performed at the Analytical Imaging Facility of the Albert Einstein College of Medicine.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
early embryonic cell
Cellular Component
cleavage furrow
Rosana Leonard
Dustin Grzesik
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
single-spot confocal microscopy
Image Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
visualization of contiguous regions
Processing History
fast Fourier filtered
contrast adjustments
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 1024px 0.154316µm
Y 1024px 0.154316µm
Z 231px 0.284923µm