Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35619^* Cite

Description

Actin fiber organization in primary keratinocyte. There are numerous actin arcs around the periphery of the cell and actin bundles that terminate in focal adhesions (bright structures perpendicular to edge).

Technical Details

Image collected on a Zeiss Axiovert 200M microscope using a 63X 1.4 NA objective. Image was contrast stretched using Image J.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: keratinocyte
Cellular Component: actin cytoskeleton; focal adhesion

Biological Context

Biological Process: cytoskeleton organization

Attribution

Names: Ann Wheeler
OTHER: University London, Blizard Institute Advanced Microscopy Facility

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL35619
Archival Resource Key (ARK): ark:/b7295/w9cil35619

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: Alexa Fluor 488; phalloidin
Processing History: unprocessed raw data

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	390px	0.2µm
Y	457px	0.2µm