Alternate header for print version


Licensing
This image is copyright protected. Any public or private use of this image is subject to prevailing copyright laws. Please contact the content provider of this image for permission requests.
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36027*  Cite 
Description

Shown is a fibroblast from a Hutchinson-Gilford Progeria syndrome patient which carries a truncation in the gene for lamin A (red, left image), a major component of the nuclear lamina. The distribution of lamin B is seen in the center image (green) and the merge at right. This passage 26 cell shows marked nuclear envelope abnormalities especially in the distribution of lamin A. Other nuclear envelope abnormalities observed in HGPS cells are shown in other images in this group. See Fig 1 in R.G. Goldman et al. 2004 Proc Natl Acad Sci 101:8963-8968.

Technical Details

Cells were fixed in formaldehyde or cold methanol, processed for indirect immunofluorescence and images recorded using a Zeiss LSM510 confocal microscope.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
AG11513
HGADFN003
AG09602B
Cellular Component
nuclear envelope
Attribution
Names
RD Goldman
DK Shumaker
MR Erdos
M Eriksson
AE Goldman
LB Gordon
Y Gruenbaum
S Khuon
M Mendez
R Varga
FS Collins
Published
R.D. Goldman et al. 2004. Proc Natl Acad Sci 101:8963-8968
Pubmed
15184648
Link
nuclear envelope
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36027
Archival Resource Key (ARK)
ark:/b7295/w9cil36027
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Fluorescein (FITC)
Rhodamine
Alexa Fluor 488
Alexa Fluor 568
Processing History
montage
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
coagulating-fixative fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 782px ——
Y 248px ——