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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36184*  Cite 
Description

Development of cytoskeletal polarity in cultured hippocampal neurons after 15 days in vitro. Neurons were immunostained for MAP2, a microtubule associated protein localized to dendrites (red) and tubulin, which is present in both axons and dendrites (green).

Technical Details

Embryonic rat hippocampal neurons were prepared as previously described (see Kaech and Banker, 2006, Nat Protoc). Cells were prepared for fluorescent staining as previously described (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Briefly, cells were fixed (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4), permeabilized with 0.25% Triton and immunostained for MAP2 (polyclonal Ab266, S. Halpain, with DyLight549 conjugated secondary, excitation, 555, emission, 568, [Jackson Immunoresearch]) and tubulin (DM1A, Sigma with Alexa 488 conjugated secondary, excitation, 494, emission, 519 [Invitrogen, Molecular Probes]). Images were acquired with a Leica DMRA microscope with a 20X lens (Fluotar, NA 0.5), Photometrics CoolSnap ES CCD camera and MetaMorph software. This color was generated using the MetaMorph "color combine" function.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
multipolar neuron
Cellular Component
dendrite
microtubule cytoskeleton
axon
synaptic vesicle
Attribution
Names
Ginger Withers (Whitman College)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36184
Archival Resource Key (ARK)
ark:/b7295/w9cil36184
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1300px 0.339µm
Y 1030px 0.339µm