Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36594^* Cite

Description

4D video of zebrafish embryo mitosis and cytokinesis. This time lapse video of z-series images was taken of a zebrafish embryo expressing EMTB-3XGFP (a microtubule marker) taken shortly after fertilization.

Technical Details

Images were collected on a Zeiss LSM 710 with a 20X 1.0 NA objective.

Biological Sources

NCBI Organism Classification: Danio rerio
Cell Type: embryo
Cellular Component: microtubule; spindle; aster

Biological Context

Biological Process: mitosis; cytokinesis after mitosis

Attribution

Names: Martin Wuehr; Timothy Mitchison

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36594
Archival Resource Key (ARK): ark:/b7295/w9cil36594

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2048px	0.2595µm
Y	2048px	0.2595µm
Z	10px	4.915µm