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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36627*  Cite 
Description

The cytoplasm of P. multimicronucleatum like all other cells contains a large number of organelles that are membrane bound. Membranes are used to compartmentalize and isolate metabolic and enzymatic processes within a continuous cytosol and cytomatrix which is the background phase of the cytoplasm. The principle site of biogenesis for secretory proteins, lysosomal hydrolases and membrane lipids and proteins is the endoplasmic reticulum, a system of membrane cisternae and tubules. This quick-freeze deep-etch preparation of a living cell shows an extensive fracture through the ER showing its P-fracture face. Intramembrane particles (IMPs) form clumps and patterns rather than being evenly spread within the membrane. Some of these IMPs probably represent sites of ribosome attachment to translocons. Translocons are intramembrane pores through which proteins are translocated into the ER during their translation. The flattened ER cisternae morph into tubular ER. TEM taken on 6/5/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytoplasm
endoplasmic reticulum
mitochondria
Biological Context
Biological Process
cytoplasm organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36627
Archival Resource Key (ARK)
ark:/b7295/w9cil36627
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
elevation
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
freeze_fracture/freeze_etch
shadowing and plating
recorded image
film
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2474px ——
Y 3000px ——