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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36631*  Cite 
Description

Occasionally mitochondria of Paramecium become filled with cristae that display a wavy or helical pattern. In thin sections these cristae are viewed as curved arcs as well as cross sectioned rows in different parts of the organelle. Stacks of decorated tubules of the contractile vacuole system are also seen in this micrograph. Cristae of mitochondria bear F1F0 ATP synthases while the decorated tubules bear the related V1V0 V-ATPase proton pump. The helical arrayed F1F0 and V1V0 units may be responsible for forming the tubular nature of the cristae and the tubular decorated spongiome (for discussion of this hypothesis see Allen, Protoplasma 189:1-8, 1995). TEM taken on 5/6/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 14,250X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
mitochondrion
contractile vacuole
integral to contractile vacuolar membrane
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36631
Archival Resource Key (ARK)
ark:/b7295/w9cil36631
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 1088px ——
Y 1236px ——