Paramecium also contains many crystals in older cultures. This micrograph is of a crystal taken from a cell in stationary phase (day 8 post-inoculation). Crystals are enclosed by a membrane and contain sheets of paracrystalline material similar to that in lysosomes and phagolysosomes that lie just inside the membrane. The crystal membrane has a pronounced glycocalyx like phagolysosomes and lysosomes and may store digestive enzymes in a paracrystalline form during periods of starvation or limited food supply. TEM taken on 3/6/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Bar = 0.5µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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