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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36640*  Cite 
Description

Paramecium also contains many crystals in older cultures. This micrograph is of a crystal taken from a cell in stationary phase (day 8 post-inoculation). Crystals are enclosed by a membrane and contain sheets of paracrystalline material similar to that in lysosomes and phagolysosomes that lie just inside the membrane. The crystal membrane has a pronounced glycocalyx like phagolysosomes and lysosomes and may store digestive enzymes in a paracrystalline form during periods of starvation or limited food supply. TEM taken on 3/6/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,250X. Bar = 0.5µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
crystalloid
cytoplasm
Biological Context
Biological Process
cytoplasm organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36640
Archival Resource Key (ARK)
ark:/b7295/w9cil36640
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2740px ——
Y 3000px ——