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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36668*  Cite 
Description

In quick-freeze deep-etch preparations in which cells are slam frozen the pellicle and the plasma membrane tends to be flattened. The double rings are present but only the outer ring of IMPs stand out prominently. The inside ring seems embedded more deeply into the plasma membrane’s P-face, i.e., into the cytosolic leaflet of this membrane. Three sets of rings are seen in this plate along with a parenthesis-shaped ring which is present at a docking site lacking a trichocyst. Pits appear in the rings where the rosettes were removed that remained attached to the E-face of the plasma membrane. TEM taken on 3/6/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
plasma membrane
trichocyst
pellicle
Biological Context
Biological Process
plasma membrane organization
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36668
Archival Resource Key (ARK)
ark:/b7295/w9cil36668
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
elevation
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
freeze_fracture/freeze_etch
shadowing and plating
recorded image
film
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2166px ——
Y 2652px ——