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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36713*  Cite 
Description

The postoral fibers are also heavily labeled by anti-b-tubulin. The cell was lightly fixed, placed in metylcellulose and frozen in liquid nitrogen. Sections were produced at -100°C before thawing and treated with the b-tubulin antibody followed by immunogold labeled antibody to IgG. TEM taken on 2/25/94 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Bar = 0.5µm.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
oral apparatus
cortical microtubule cytoskeleton
Attribution
Names
Richard Allen (University of Hawaii)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36713
Archival Resource Key (ARK)
ark:/b7295/w9cil36713
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2130px ——
Y 2178px ——